Development of New Extracts of Crocus sativus L. By-Product from Two Different Italian Regions as New Potential Active Ingredient in Cosmetic Formulations

This project aimed to apply eco-friendly extraction methods Crocus sativus L. by-product (flowers without stigmas i.e., tepals composed of petals and sepals) recover extracts with high antioxidant capacity polyphenol content, be used in cosmetic products. Flowers grown two different Italian regions (Sample 1—Alba Piemonte, north Italy Sample 2—Sibillini Marche, centre Italy) were subjected for the first time microwave-mediated green solvents extractions (MGSE) andquali-quantitative determination molecules. Firstly, from 1 selected according their total phenol content (TPC) by Folin–Ciocalteu’s assay (AC) spectrophotometric assays. Then, preliminary results, MGSE carried out ethanol 70°, water, glycerin as most performing applied both Samples 2. The best results obtained using solvents, such water or samples coming Marche. identification quantification phenolic compounds, belonging anthocyanins flavonols classes, was performed UPLC-DAD-ESI-MS. Concerning abundant analyte is kaempferol 3-O-sophoroside extract showed higher amount flavonols, reaching concentration 25.35 mg 3-O-glucoside equivalent per gram DW raw material. Among anthocyanins, delphinidin 3,5-O-diglucoside anthocyanin detected extract. Two new myricetin-di-glucoside primflasine, identified high-resolution mass spectrometry (HRMS). batches thus characterized evaluated biological potential safety keratinocyte HaCaT cells. not cytotoxic up 0.03 mg/mL. 70° effective counteracting oxidative stress induced H2O2 UVA exposure reduced cytotoxicity UVB exposure. also able significantly reduce sodium dodecyl sulphate-induced damage. Taken together, these suggest a use waste materials cosmeceutical preparations antiaging, anti-skin irritation formulation by-products.